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Literature summary extracted from
- Ser475, Glu272, Asp276, Asp327, and Asp360 are involved in catalytic activity of human tripeptidyl-peptidase I (2005), FEBS Lett., 579, 1383-1388.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.4 | expression in CHO cells | Homo sapiens |
| 3.4.14.9 | expression of wild-type and mutant enzymes (E272A, D276A, D327A, D360A and S475L) in CHO cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.11.4 | D276A | kcat/KM is 21% of wild-type value | Homo sapiens |
| 3.4.11.4 | D327A | kcat/KM is 6% of wild-type value | Homo sapiens |
| 3.4.11.4 | D360A | kcat/KM is 3% of wild-type value | Homo sapiens |
| 3.4.11.4 | E272A | kcat/KM is 3% of wild-type value | Homo sapiens |
| 3.4.11.4 | S475L | kcat/KM is 0.4% of wild-type value | Homo sapiens |
| 3.4.14.9 | D276A | kcat/Km is 21% of the wild-type value | Homo sapiens |
| 3.4.14.9 | D327A | kcat/Km is 6% of the wild-type value | Homo sapiens |
| 3.4.14.9 | D360A | kcat/Km is 3% of the wild-type value | Homo sapiens |
| 3.4.14.9 | E272A | kcat/Km is 3% of the wild-type value | Homo sapiens |
| 3.4.14.9 | S475L | kcat/Km is 0.4% of the wild-type value | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.4 | 0.076 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme S475A | Homo sapiens |  |
| 3.4.11.4 | 0.098 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, wild-type enzyme | Homo sapiens | |
| 3.4.11.4 | 0.105 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D276A | Homo sapiens | |
| 3.4.11.4 | 0.111 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D360A | Homo sapiens | |
| 3.4.11.4 | 0.118 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme E272A | Homo sapiens | |
| 3.4.11.4 | 0.164 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D327A | Homo sapiens | |
| 3.4.14.9 | 0.076 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme S475L | Homo sapiens | |
| 3.4.14.9 | 0.098 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, wild-type enzyme | Homo sapiens | |
| 3.4.14.9 | 0.105 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D276A | Homo sapiens | |
| 3.4.14.9 | 0.111 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D360A | Homo sapiens | |
| 3.4.14.9 | 0.118 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme E272A | Homo sapiens | |
| 3.4.14.9 | 0.164 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D327A | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.4 | lysosome | - |
Homo sapiens | 5764 | - |
| 3.4.14.9 | lysosome | - |
Homo sapiens | 5764 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.4 | Homo sapiens | - |
- |
- |
| 3.4.14.9 | Homo sapiens | O14773 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.4 | - |
Homo sapiens |
| 3.4.14.9 | - |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.4 | Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.14.9 | Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.4 | TPP I | - |
Homo sapiens |
| 3.4.11.4 | tripeptidyl-peptidase I | - |
Homo sapiens |
| 3.4.14.9 | tripeptidyl-peptidase I | - |
Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.4 | 0.04 | 1.97 | Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D276A | Homo sapiens | |
| 3.4.11.4 | 0.12 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme S475A | Homo sapiens | |
| 3.4.11.4 | 1.52 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D360A | Homo sapiens | |
| 3.4.11.4 | 1.54 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme E272A | Homo sapiens | |
| 3.4.11.4 | 3.22 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, wild-type enzyme | Homo sapiens | |
| 3.4.11.4 | 4.22 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D327A | Homo sapiens | |
| 3.4.11.4 | 9.36 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D276A | Homo sapiens | |
| 3.4.11.4 | 41.66 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, wild-type enzyme | Homo sapiens | |
| 3.4.14.9 | 0.04 | 1.97 | Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D276A | Homo sapiens | |
| 3.4.14.9 | 0.12 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme S475L | Homo sapiens | |
| 3.4.14.9 | 1.52 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D360A | Homo sapiens | |
| 3.4.14.9 | 1.54 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme E272A | Homo sapiens | |
| 3.4.14.9 | 3.22 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, wild-type enzyme | Homo sapiens | |
| 3.4.14.9 | 4.22 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D327A | Homo sapiens | |
| 3.4.14.9 | 9.36 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, mutant enzyme D276A | Homo sapiens | |
| 3.4.14.9 | 41.66 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 5.0, 37°C, wild-type enzyme | Homo sapiens | |
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